Insertion junction: CLIP2.014760_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014760
Chromosome:chromosome 10
Location:5719904
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g459550 B9D2B (1 of 1) PTHR12968:SF2 - B9 DOMAIN-CONTAINING PROTEIN 2; B9 Domain-Containing transition zone protein 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGATTCAACCACTCCGTCCTCCACGCACACTATCCACCCCCCCCCCC
Internal bar code:TGTCTCCATCTGTGGCTTGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:563
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGCTAACATGCACATGC
Suggested primer 2:CCTCTCTCTCTCCCTTCCCC