| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014776 |
| Chromosome: | chromosome 2 |
| Location: | 8477709 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142000 | CrUbcE2_1,CrUbc9,CreSCE1a,CrSCE1,UBC4,SCE1 | (1 of 1) PTHR24067//PTHR24067:SF153 - UBIQUITIN-CONJUGATING ENZYME E2 // SUBFAMILY NOT NAMED; SUMO-conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGACACAGGACCCCCCGTGCACGACCGCCCTTCCCGGCCTCAGCCCG |
| Internal bar code: | ATCCAATGCGGGCTGTTGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1375 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGCTCGACCAGAAACC |
| Suggested primer 2: | GCAGCTGCTTGATGGTGATG |