Insertion junction: CLIP2.014786_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.014786
Chromosome:chromosome 4
Location:2024656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219150 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGGGGAACAGGGACCCGAACGAGAAATATCCCAACGACCCAAACACC
Internal bar code:TATAAGCGCGTATCCTCTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1764
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGCATTGTCGGCAATTAT
Suggested primer 2:GTTCTGCTGTTCCCTACCCC