| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014797 |
| Chromosome: | chromosome 10 |
| Location: | 5489797 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457750 | SULTR1 | (1 of 2) K18059 - sulfate transporter 4 (SULTR4); Sulfate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCTCAAGTATGCGAGTAACGGCGCCCCGACTGATTACCACACCTGCC |
| Internal bar code: | TCATTGTACGCTATAAGTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4471 |
| LEAP-Seq percent confirming: | 95.082 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGCTGCTTTTCCTTGG |
| Suggested primer 2: | AATATGTTGGGACAGCGGCA |