| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014805 |
| Chromosome: | chromosome 3 |
| Location: | 6029468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189850 | (1 of 1) IPR000253//IPR001357//IPR008984 - Forkhead-associated (FHA) domain // BRCT domain // SMAD/FHA domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGCTGCTCAGCCGGGCAACAGCTGCCAGCGCTACGGCGCGTGGCGCG |
| Internal bar code: | TGGGTTGTAGGCAGTAATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5744 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGGTCCTTACTTGGCTG |
| Suggested primer 2: | GGTAGTGACGGTTGCTGACA |