Insertion junction: CLIP2.014817_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014817
Chromosome:chromosome 5
Location:941106
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGCCGCCACCGCCGCCACCACGTGTGCCGCCCGCACCATCTGGTACC
Internal bar code:TGATAGTCATCCATTGTCTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:0.952381
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:104
LEAP-Seq n unique pos:105

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTGTGGATATGAGGTCG
Suggested primer 2:GTGTTGGACGGAACTTTGCC