Insertion junction: CLIP2.014888_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014888
Chromosome:chromosome 3
Location:5098791
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g180450 FPN1,FAP215 (1 of 3) K01081 - 5'-nucleotidase (E3.1.3.5); Flagellar Associated Protein 215, 5'-nucleotidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGGTAGGGTCTCTGCGTGGGGTATAGCGCCTGCTTAGGTTTGCCGCAT
Internal bar code:ACGGTTGGATAACTGGATGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:5599
LEAP-Seq percent confirming:99.1736
LEAP-Seq n confirming:120
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:121

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTACTCCATGTCCGCCAC
Suggested primer 2:CAACCCGCTTGTCCTCTTCT