| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.014896 |
| Chromosome: | chromosome 3 |
| Location: | 3801595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169850 | TSC1 | (1 of 1) PTHR10890:SF3 - CYSTEINE--TRNA LIGASE, CYTOPLASMIC; Cysteinyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCGACACTACCCCACGGATGGCAAGGGGCACAGTCCTACCTGTATT |
| Internal bar code: | GGTAGATTCAATCGTCCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1571 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACTAGCCCCTCCCAACT |
| Suggested primer 2: | AGCACTCTTGTGGACATGGG |