| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.014966 |
| Chromosome: | chromosome 13 |
| Location: | 3297457 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTCGCCTGACGTCGATGCTGGTGGATGCAAGTGCGGCGGGCAGCCTC |
| Internal bar code: | TGGATTCAGCACCTTGCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1447 |
| LEAP-Seq percent confirming: | 94.2029 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTGCATGTGGTTGAAA |
| Suggested primer 2: | GAGATTGCCCTACTCGGACG |