Insertion junction: CLIP2.014993_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014993
Chromosome:chromosome 16
Location:2656861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g661500 (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGCGCGCTCCTGGGCTGCGAAGGGTCTCATTATGCTTCAAGGTCCCGT
Internal bar code:CTCGTGGGCGGGGTAAGTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:5583
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCTCGGTAGCGATGATGC
Suggested primer 2:CTCAGTTCCCAGCCGAAGTT