| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014995 |
| Chromosome: | chromosome 3 |
| Location: | 4330323 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174500 | HDZ1 | (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTATTTAGGTGTGTTACTTAAGCAGAGGGGTGTGCCGACGCAGCGA |
| Internal bar code: | TAATGGTCGATCCGAGTGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 24.2424 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACTCCCCTGTCCATGAA |
| Suggested primer 2: | AACTACACATCGCAGCGTCA |