Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.014997 |
Chromosome: | chromosome 1 |
Location: | 739162 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCCTCAATGCGTCGCGCGCCGCTCTGGCTGACCCCCAGCACATCGAT |
Internal bar code: | GTCCTTCCTTAACGCACTCTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1682 |
LEAP-Seq percent confirming: | 95.6522 |
LEAP-Seq n confirming: | 22 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGCTGTCACCACACCTTT |
Suggested primer 2: | CACCAGAAGTTGCACATGGC |