Insertion junction: CLIP2.015006_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015006
Chromosome:chromosome 3
Location:908140
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g146567 (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCGGACCTGGCATCAGCCTGCCACGGCGCAACGCCCGCTGTGGTCGC
Internal bar code:AGGACGTACTGTTTTATTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:783
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACTAGCCCTAGTCCCGT
Suggested primer 2:CATATGGCGCTGGGATGGAT