Insertion junction: CLIP2.015010_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.015010
Chromosome:chromosome 2
Location:2462494
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g091700 RIB72 Flagellar protofilament ribbon protein 72; (1 of 1) PF06565//PF13499 - Repeat of unknown function (DUF1126) (DUF1126) // EF-hand domain pair (EF-hand_7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTACTACCTCGAGGACGACAGCGTGGAGGTGCTGGAGATCAACGAGAA
Internal bar code:TGAGGAATGGAAGCGGGTGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3759
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCACCATCTTCACCACG
Suggested primer 2:TTGGATGTGGAATGGGGAGC