| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015017 |
| Chromosome: | chromosome 12 |
| Location: | 6929158 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561602 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCTGCCAGAGTGCCGGCAGTGTACCGCAAGCCCACTGGCACGGAGCC |
| Internal bar code: | TGCCATGTAACTGTGTCAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1501 |
| LEAP-Seq percent confirming: | 82.8125 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCGGAGTAGATGTCGGC |
| Suggested primer 2: | CGGGTACAGGTATACGGTGC |