Insertion junction: CLIP2.015043_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015043
Chromosome:chromosome 12
Location:6461276
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g537550 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGCGCAGCCGCCAGCACCGCCCGGTAGTCGGACACCTGCACGGGAG
Internal bar code:CATGTTTGTCAGGACACATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1608
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GATGGGGAAGGGTGGGAAAG
Suggested primer 2:TCCCATACACACACACACGG