Insertion junction: CLIP2.015047_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.015047
Chromosome:chromosome 2
Location:2689057
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093401 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAGCACTATGGTAACTCACCCAAGTATAAAACGTCCACAATCTGGCG
Internal bar code:GGGTATGTTAGAATCTAAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1432
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGGACTGGGGTAGGTACT
Suggested primer 2:CTGTCGGCCAAGTCTTTTGC