Insertion junction: CLIP2.015057_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015057
Chromosome:chromosome 2
Location:3245611
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097200 SRR14 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGCCTCGCGCTCCTGCTGCCGGCTGATCAGCAGAACCTGCGTGGTG
Internal bar code:TTATAGTGAGTTAGCCGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1288
LEAP-Seq percent confirming:97.5
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGGCGTGACGTGTTTGT
Suggested primer 2:GGGACTTCTCCTCTCCACCT