Insertion junction: CLIP2.015058_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015058
Chromosome:chromosome 2
Location:5572107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g115350 (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGTCCGCGCACACGGCCATGACAACGCGGTGTTTGAGTTCACCATCAC
Internal bar code:TAATGAACGCGACCTAGGAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2206
LEAP-Seq percent confirming:91.8919
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCAGTTGGCGTCCGGAAT
Suggested primer 2:TGCGAAACGGTGTGGTGATA