| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.015081 |
| Chromosome: | chromosome 2 |
| Location: | 8438351 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142352 | TSH1 | (1 of 1) PTHR11476:SF7 - HISTIDYL-TRNA SYNTHETASE, ISOFORM D; Putative histidinyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCTAAGCGCCACCGCCCCCCTTGTCATCTCCTGCTGATGCGCAGCGG |
| Internal bar code: | AGTGCCTTAAAAATGTTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTTTGCATACGGATGAG |
| Suggested primer 2: | CTAGCACCAAGGCCTACGAG |