| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.015091 |
| Chromosome: | chromosome 9 |
| Location: | 50889 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGAGTTCAAGGCTTTGCGAGGGCTAAAAGCAGCTCTTCGTGCACATT |
| Internal bar code: | GCACTCAGGAGCTTTATAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1136 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTCGTAAGTAGGCCAT |
| Suggested primer 2: | TTCTCCCTCTCTCTCCAGCC |