Insertion junction: CLIP2.015112_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015112
Chromosome:chromosome 3
Location:5359492
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183300 (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCCGCCATAGGGCGCAGCACGACGAGGTCAGCGAGCCGCGCGCCACAC
Internal bar code:TCATGCCTATGCGGCAATCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4410
LEAP-Seq percent confirming:23.913
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTCATTCTGCATCGTTG
Suggested primer 2:CAACTACGGCGCTACATTGC