| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015131 |
| Chromosome: | chromosome 14 |
| Location: | 3593174 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630895 | (1 of 1) PTHR13789:SF90 - FAD-BINDING MONOOXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCACTGCCTGCAACTTGAGTATCACGACACCCACATCCCGTGTTCCAG |
| Internal bar code: | AAAATTAGCTAGGTACCGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACACCCTATCCAACCC |
| Suggested primer 2: | GTACTCGTGGGTGGGTGAAG |