| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.015136 |
| Chromosome: | chromosome 9 |
| Location: | 1951610 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801010 | (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCGTGTGCATGTCGCGGCATGTGGATGTAGAGCAACTGAGGCTGTCT |
| Internal bar code: | GTAAGAACAGCACGGCATCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3646 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCATCCCCAAAGATCC |
| Suggested primer 2: | GGGGGAGGTCATTGTCAAGG |