| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015148 |
| Chromosome: | chromosome 7 |
| Location: | 2620638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATATAAGCCCGAAGCTTCTCTCCCGTCGTCTCCCAGCTCAGCCCGCC |
| Internal bar code: | GCGGGTGTTCTAGTGAGGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCACATGATGACTGAAG |
| Suggested primer 2: | TAATAGCTGCCCTGCATCCG |