Insertion junction: CLIP2.015152_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015152
Chromosome:chromosome 17
Location:3630135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g725350 PSL4,GLC2B Glucosidase IIb; (1 of 1) K08288 - protein kinase C substrate 80K-H (PRKCSH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGAAAGGTCGGCGCGGGCGTGGGGTGTTGGGAGTCCGGGGCACCACC
Internal bar code:ATGTGGATGGGGGTGTACTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2132
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGCCCTAAACGCTAATG
Suggested primer 2:CCACCACTAAGGAGCACCAG