Insertion junction: CLIP2.015169_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015169
Chromosome:chromosome 2
Location:7169412
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390467 CGL66 (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN; Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCCCGACAGCCGCGCCCCAGCCGGCCCTTCCCCCACTCCGGCGACAA
Internal bar code:GTGAGCGTGAGGGGGCGATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:453
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTGTGTTTGTGCG
Suggested primer 2:CGTATCGGGCAGCAAAATGG