| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015180 |
| Chromosome: | chromosome 16 |
| Location: | 7530126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683035 | (1 of 2) IPR003593//IPR027417 - AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGTCGTGCCTGTCAAAGTAGCGCAGCTGCGTGTGGGGACCTGCCTTG |
| Internal bar code: | GTAGGCAATAGGCGCTCATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2221 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCACGTTCCTTGTGTGG |
| Suggested primer 2: | GTGCTACGCAACAAACAGCA |