| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015191 |
| Chromosome: | chromosome 3 |
| Location: | 2639958 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160600 | (1 of 1) IPR000104//IPR000679//IPR013088 - Antifreeze protein, type I // Zinc finger, GATA-type // Zinc finger, NHR/GATA-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTTCAAATCTGTATTTCGCTTTCCTATGCCACAGGCTCACCCACCGC |
| Internal bar code: | GTCAGCACGGCTGCCGTAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4399 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCATGACCAAAGTGTG |
| Suggested primer 2: | TACACTCGCATGGATCGACG |