Insertion junction: CLIP2.015194_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015194
Chromosome:chromosome 2
Location:1241773
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g081850 (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACTGTGCCACAACACAACGAGCTCAGGACCGCGGCTTACAGCCCGAT
Internal bar code:TTTGTTTAGTGATTTTGCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:7041
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTACGCTGAAGTCAAGCA
Suggested primer 2:CTCTGCCAGGAACCTGTGAG