Insertion junction: CLIP2.015200_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015200
Chromosome:chromosome 17
Location:3139031
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTTTCCGTGGCGGCTAACGCTAAAAACTAAGTTCCTATTGAAAGCA
Internal bar code:AAAACGGGCGTTTAGCATTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2088
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAACACTTCCTCGCCTCG
Suggested primer 2:CCCTTTTGCCTTTGGTTCGG