| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015203 |
| Chromosome: | chromosome 12 |
| Location: | 8564293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548900 | ROC23,ROC112 | (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCATGTTTCCCGGGGCACCTAAATGCGGGCCCGCCGGTTGATAGA |
| Internal bar code: | CGTGCGAAGTCTGCTTTACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTTCATGGATCGCTTG |
| Suggested primer 2: | GGGGAGACAAGAAGGACAGC |