Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.015210 |
Chromosome: | chromosome 3 |
Location: | 1321258 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g150400 | PIGU1,PIGU,LRG5 | GPI transamidase subunit PIG-U; (1 of 1) K05293 - phosphatidylinositol glycan, class U (PIGU) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACTGCCGGGTGCCCACCTCTCGGCCCACCCGCCGCTCCCTCCGTGC |
Internal bar code: | TGAGTATTTATGGCTGCATTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2933 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACAGTTTACAGACGGGCC |
Suggested primer 2: | AAAACACCACCCCCGAAACT |