Insertion junction: CLIP2.015213_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015213
Chromosome:chromosome 9
Location:3230524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394732 AZI1,MOT3,CEP131 Centrosomal Protein 131; (1 of 1) K16540 - 5-azacytidine-induced protein 1 (AZI1, CEP131) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCCAATCACAATCGGCCTGCGCGCACAAGAGCCATCGCGCTGGCGGCC
Internal bar code:ACAGACAGTAATGAGACTGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1709
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGCATTGTTAGCGCTAC
Suggested primer 2:AACAGTGACAGCCCTTCGAG