Insertion junction: CLIP2.015216_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015216
Chromosome:chromosome 5
Location:829523
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246050 OTU3 (1 of 1) K13717 - OTU domain-containing protein 3 [EC:3.4.19.12] (OTUD3); OTU-like cysteine protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACTCGACCAATGTGCCCGCACTGCACCGCACCGCACCGCTTGCAGGCA
Internal bar code:ATTTGTTCCCGACTGGTCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGTTGGGGGAGTGAAGG
Suggested primer 2:CTGGTGATGCGCATGACATG