Insertion junction: CLIP2.015225_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015225
Chromosome:chromosome 3
Location:4431417
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g175351 LMR2 (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACACCAGGAAGGCGACGCTTGGAAGACCGAAGAACGACACGACGTTCT
Internal bar code:TTTTAGATCTCACTGTTGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:98
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTCCACGTCGTGACAGA
Suggested primer 2:ATCCCGGCAGTACTGGTACT