Insertion junction: CLIP2.015252_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015252
Chromosome:chromosome 9
Location:2684501
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801022 (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGATAGCTCGCTCGCCCGAGCTCGCAATACCAATAATAATAAACTGCC
Internal bar code:AACCCGCATTTACCCAAGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1067
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGATGACGGAGCAGTAC
Suggested primer 2:CATGACGTATGCCGCTAGGT