| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015267 |
| Chromosome: | chromosome 6 |
| Location: | 1103276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257600 | FA1 | (1 of 4) PF13516 - Leucine Rich repeat (LRR_6); Flagellar Autonomy Protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCCAACGCAAATACCGCAAGTGCAGGGTTGGGGGCGCGATACGGCTC |
| Internal bar code: | TGTCCATGTCGTCTGGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2169 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCACATGCCCGTGTAACC |
| Suggested primer 2: | GTGTTGGACCTGGAGGGATG |