Insertion junction: CLIP2.015315_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015315
Chromosome:chromosome 3
Location:4677584
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g177250 HAP3 (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase; Haloperoxidase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGGGGCATCAGCGACAGCCAGGTGCCGCTCTACCAGAAGCTGATCGT
Internal bar code:GCACGGCCGTGATAATGATCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1041
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAACAGCGGTAAGGACA
Suggested primer 2:CAATCGACCGTCATTGCGAC