Insertion junction: CLIP2.015345_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015345
Chromosome:chromosome 12
Location:9786201
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549427 (1 of 1) 1.17.4.2 - Ribonucleoside-triphosphate reductase / Ribonucleotide reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTTAATAGTGGTCCTCGGCCCCCCCCCCCCAGCACACACACACACACA
Internal bar code:TTTATGGCCGAACTCATTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCGAATGTCCTGAACCC
Suggested primer 2:AATCTGTGTTGCGGGAGAGG