| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015378 |
| Chromosome: | chromosome 5 |
| Location: | 3039276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231100 | (1 of 1) PF00023//PF12796//PF13637 - Ankyrin repeat (Ank) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCTGGTTGGTGGTGGCTGGTGCTCCCGTACATTCCGTACGTACTCGG |
| Internal bar code: | AAACGTGTGTGCTCAGGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4129 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTTCTGCCATCCCTTC |
| Suggested primer 2: | GTTGCCACTTGCCAGTTGAG |