| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015392 |
| Chromosome: | chromosome 2 |
| Location: | 3649947 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099950 | (1 of 6) 3.4.19.12//3.4.22.69 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase // SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTGCACTCACCATCATCGTCCTCATCCTCATCCTCATCATCATCATC |
| Internal bar code: | AGTTTGTTCCGCTTTAGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2993 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAAGCACCTCGTTGTAC |
| Suggested primer 2: | ACGAATGCGGTCAGTGTAGG |