Insertion junction: CLIP2.015396_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015396
Chromosome:chromosome 16
Location:301690
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATTCCCTGATTCCCTGATCCGCGGGGCGCGGGCACAGCCCAAAGACC
Internal bar code:GGCGACGCTTTATTGACATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGTGTGGACATGC
Suggested primer 2:CGTTGGGGGCTGAGTGTATT