Insertion junction: CLIP2.015420_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015420
Chromosome:chromosome 2
Location:4652031
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107550 CVL2 Fe2+/Mn2+ transporter, VIT1/CCC1 family; (1 of 2) PF01988 - VIT family (VIT1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCTGCGCCATACCTGGTGTCCCGCAACATAACTCCGAGTCCGCCACC
Internal bar code:TGGGGCAAATGAAGACAGACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:6181
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCATGACACACAGGGATG
Suggested primer 2:AAGGGCAAGGGTTCATACCG