Insertion junction: CLIP2.015429_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015429
Chromosome:chromosome 3
Location:498618
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145027 (1 of 14) PF13371 - Tetratricopeptide repeat (TPR_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTCGCACTGCCTCGCCGGCGCGCTGGCACCATCATGCGACCGTGACCG
Internal bar code:CGCAACCTACGAATATATTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1285
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACCCTCACAAACCGCAG
Suggested primer 2:TTCCGCTCGCTCATCATCTC