Insertion junction: CLIP2.015453_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015453
Chromosome:chromosome 1
Location:2162166
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g012100 ARS4 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACACCCACTGCCCTCCTGTCCCACACACCAATCGGGCGAGCGGCAC
Internal bar code:GGCGCACTAGTCACTGACAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGCGGTAAGTGGGGAAG
Suggested primer 2:CAAGGGCTTCACCGTGTTTG