Insertion junction: CLIP2.015454_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015454
Chromosome:chromosome 15
Location:343970
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635400 ZYS3,ZYS3-1 (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Zygote-specific protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAGTTCAGCCACAGCGCGGAGCCGTCGGCGGCGGTGACGCGGGCCCA
Internal bar code:GCTGCGCCTTAGATCACAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCATCCCCTCCTTCA
Suggested primer 2:CCGGTCTCCACGTTGAAGAA