Insertion junction: CLIP2.015469_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015469
Chromosome:chromosome 9
Location:3103403
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394102 GUK2 Guanylate kinase; (1 of 1) 1.1.1.216//2.7.4.8 - Farnesol dehydrogenase // Guanylate kinase / Guanosine monophosphate kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAAACATACGGACGCCGTGGATGGTAGGGAACTATAGACGCACCGAG
Internal bar code:ATGGAACCAAACGCTACAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3160
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATCTTCGTGTTCATCGC
Suggested primer 2:CGCACATGAACAAGCACACA