Insertion junction: CLIP2.015485_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015485
Chromosome:chromosome 1
Location:874413
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004950 (1 of 2) 4.2.1.130 - D-lactate dehydratase / Glyoxylase III; Conserved Protein with Putative intracellular protease/amidase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCCCACAGCTGGTTCCGTTCCTGCTGGAGTCCAGGCTGCGTGAGCAG
Internal bar code:GAAATGGACAAGAGCTGAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACGCCAATCACACACA
Suggested primer 2:TCATCGTGCACAGAAACCGA