Insertion junction: CLIP2.015487_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.015487
Chromosome:chromosome 2
Location:1289271
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g082350 CUTA1,CUT1 (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATCCCTTGAGCCAACGTGCATCCCAACGCCGCCCGCGCCGACCCCACA
Internal bar code:ATCGATTCATCATGAAAGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2308
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCATAGACGCACCGAAGC
Suggested primer 2:CCCTGTTGGCGTTGTTTAGC